Partitioning of pyrimidine single stranded oligonucleotide using polyethylene glycol - sodium sulfate aqueous two-phase systems; experimental and modeling
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- 作者：Behzad Nazera ; Mohammadreza Dehghania ; m_dehghani@iust.ac.ir" class="auth_mail" title="E-mail the corresponding author ; Bahram Goliaeib ; Elham Moradb
- 关键词：Aqueous two-phase ; Oligonucleotide ; Partitioning ; UNIFAC-FV ; Bioseparation
- 刊名：Fluid Phase Equilibria
- 出版年：2017
- 出版时间：25 January 2017
- 年：2017
- 卷：432
- 期：Complete
- 页码：45-53
- 全文大小：772 K
文摘
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Partitioning of pyrimidine oligonucleotide in ATPSs was studied.
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The effect of parameters like polymer MW, pH, temperature and were investigated.
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Addition of different second salts including KCl, KH2PO4 and MgSO4 was studied.
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99.88% of the biomolecule was extracted in the top phase at appropriate conditions.
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The partitioning was correlated using a model based on UNIFAC-FV group contribution.