Corydalis bungeana Turcz. attenuates LPS-induced inflammatory responses via the suppression of NF-κB signaling pathway in vitro and in vivo

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• 作者：Xiao-Ting Zhai^a ; ^b ; ^c ; Jia-Quan Chen^c ; Cui-Hua Jiang^a ; ^d ; Jie Song^a ; ^b ; Dong-Yu Li^a ; ^b ; Hao Zhang^a ; ^b ; Xiao-Bin Jia^a ; ^b ; Wei Tan^a ; ^b ; Shu-Xia Wang^a ; ^e ; Yi Yang^a ; ^b ; Fen-Xia Zhu^a ; ^b ; ^{zfxcjq@126.com}
• 关键词：DMSO (PubChem CID ; 679) ; NaNO2 (PubChem CID ; 23668193) ; EtOH (PubChem CID ; 702) ; Protopine (PubChem CID ; 4970) ; Corynoline (PubChem CID ; 177014) ; Acetylcorynoline (PubChem CID ; 177015) ; Dihydrosanguinarine (PubChem CID ; 124069) ; Acetonitrile (PubChem CID ; 6342) ; Triethylamine (PubChem CID ; 8471)
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：24 December 2016
• 年：2016
• 卷：194
• 期：Complete
• 页码：153-161
• 全文大小：2643 K
• 卷排序：194

文摘

Corydalis bungeana Turcz. (C. bungeana) is one of traditionally used medicines in China and possesses various biological effects, such as anti-inflammatory, antibacterial activity and inhibition of the immune function of the host.Aim of the studywe studied the anti-inflammatory effect and molecular mechanism involved of C. bungeana both in vitro and in vivo model system in which the inflammatory response was induced by LPS treatment.Materials and methodsAnti-inflammatory activity of C. bungeana was investigated by LPS-induced RAW 264.7 macrophages and BALB/c mice. The production and expression of pro-inflammatory cytokines were evaluated by Griess reagent, ELISA kits and RT-qPCR, respectively. Phosphorylation status of IκBα and p65 was illustrated by western blot assay.ResultsC. bungeana reduced the secretion of NO, TNF-α, IL-6 and IL-1β through inhibiting the protein expression of iNOS, TNF-α, IL-6 and IL-1β in vitro and in vivo. Western blot analysis suggested that C. bungeana supressed NF-κB activation via regulating the phosphorylation of IκBα and p65. Immunohistochemical assay also demostrated the histological inflammatory change in liver tissue.ConclusionsThe results indicate that C. bungeana supresses the activation of NF-κB signaling pathway through inhibiting phosphorylation of IκBα and p65, which results in good anti-inflammatory effect. In addition, C. bungeana attenuates inflammatory reaction by supressing the expression of various inflammatory cytokines both in vivo and in vitro.