Synthesis of nucleotide sugars and ¦Á-galacto-oligosaccharides by recombinant Escherichia coli cells with trehalose substrate

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• 作者：Soo-In Ryu ; Soo-Bok Lee
• 关键词：Whole-cell ; Trehalose synthase ; Trehalose ; Nucleoside diphosphate-sugar ; ¦Á-Galacto-oligosaccharides
• 刊名：Enzyme and Microbial Technology
• 出版年：2013
• 出版时间：10 October, 2013
• 年：2013
• 卷：53
• 期：5
• 页码：359-363
• 全文大小：996 K

文摘

Useful nucleoside diphosphate (NDP)-sugars and ¦Á-galacto-oligosaccharides were synthesized by recombinant Escherichia coli whole cells and compared to those produced by enzyme-coupling. Production yields of NDP-glucoses (Glcs) by whole cells harboring trehalose synthase (TS) were 60 % for ADP-Glc, 82 % for GDP-Glc, and 27 % for UDP-Glc, based on NDP used. Yield of UDP-galactose (Gal) by the whole-cell harboring a UDP-Gal 4-epimerase (pGALE) was 26 % of the quantity of UDP-Glc. ¦Á-Galacto-oligosaccharides, ¦Á-Gal epitope (Gal¦Á-3Gal¦Â-4Glu) and globotriose (Gal¦Á-4Gal¦Â-4Glu), were produced by the combination of three recombinant whole cells harboring TS, pGALE, and ¦Á-galactosyltransferase, with production yields of 48 % and 54 % , based on UDP, respectively. Production yields of NDP-sugars and ¦Á-galacto-oligosaccharides by recombinant whole-cell reactions were approximately 1.5 times greater than those of enzyme-coupled reactions. These results suggest that a recombinant whole-cell system using cells harboring TS with trehalose as a substrate may be used as an alternative and practical method for the production of NDP-sugars and ¦Á-galacto-oligosaccharides.