Lectin from seeds of a Brazilian lima bean variety (Phaseolus lunatus L. var. cascavel) presents antioxidant, antitumour and gastroprotective activities

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• 作者：Rodrigo Rodrigues e Lacerda^a ; ^{rodrigofarmapb@gmail.com} ; Edilza Silva do Nascimento^b ; ^{edilsa_nat@hotmail.com} ; José ; Thalles Jocelino Gomes de Lacerda^b ; ^{thalles_lacerda2@hotmail.com} ; Luciano da Silva Pinto^c ; ^{ls_pinto@hotmail.com} ; Caroline Rizzi^c ; ^{ccrizzi@yahoo.com.br} ; Mirna Marques Bezerra^d ; ^{mirnabrayner@gmail.com} ; Isabela Ribeiro Pinto^d ; ^{isabelaribeirosb@gmail.com} ; Samuel Mateus Pereira Filho^d ; ^{samuelmateus.ufc@hotmail.com} ; Vicente de Paulo Texeira Pinto^d ; ^{pintovicente@gmail.com} ; Gerardo Cristino Filho^d ; ^{gerardocristino@uol.com.br} ; Carlos Alberto de Almeida Gadelha^b ; ^{calbgadelha@gmail.com} ; Tatiane Santi Gadelha^b ; ^{santi.tatiane@gmail.com}
• 关键词：Lectin ; Purification ; Phaseolus ; Antioxidant ; Antitumour ; Gastroprotective
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：1072-1081
• 全文大小：1227 K
• 卷排序：95

文摘

Lectins are proteins able to interact specifically and reversibly with carbohydrates. They are present in all living beings, particularly in legume seeds, which have many biological functions. The aim of this study was to isolate, characterize and verify antioxidant, anti-hemolytic, antitumor and gastroprotective activities in a lectin present in seeds of Phaseolus lunatus L. var. cascavel (PLUN). The isolation of lectin was performed by size exclusion chromatography on Sephadex G-100, which was isolated from a protein capable of agglutinating only human erythrocytes type A, being this the only inhibited haemagglutination n-acetyl-d-galactosamine. Its weight was estimated by PAGE is 128 kDa. The lectin is thermostable up to 80 °C and is active between pH 2–11. As 8 M urea was able to denature the lectin. PLUN is a glycoprotein consisting of 2% carbohydrate and has antioxidant action with ascorbic acid equivalent antioxidant capacity (μMAA/g) of 418.20, 326 and 82.9 for total antioxidant activity, ABTS radical capture and capture of DPPH radical, respectively. The lectin has antitumor activity against melanoma derived cells at doses of 100 and 50 mg/ml, reducing up to 83% tumor cells, and gastroprotective action, reducing up to 63% damaged area of ​​the stomach induced by ethanol.