Contribution of next generation sequencing to early detection of cytomegalovirus UL97 emerging mutants and viral subpopulations analysis in kidney transplant recipients
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- 作者：Isabelle Garriguea ; b ; c ; isabelle.garrigue@chu-bordeaux.fr" class="auth_mail" title="E-mail the corresponding author ; Ré ; mi Moulinasd ; e ; f ; g ; Patricia Recordon-Pinsonb ; c ; Marie-Laure Delacoure ; f ; Marie Essigh ; Hannah Kaminskii ; Jean-Philippe Rerolleh ; Pierre Mervillei ; Hervé ; Fleurya ; b ; c ; Sophie Alaind ; e ; f ; g ; sophie.alain@unilim.fr" class="auth_mail" title="E-mail the corresponding author ; sophie.alain@chu-limoges.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：CMV ; cytomegalovirus ; GCV ; ganciclovir ; NGS ; next-generation sequencing ; PCR ; polymerase chain reaction ; PFA ; foscarnet ; Sn ; Shannon entropy ; UDPS ; ultra-deep pyrosequencing ; VGCV ; valganciclovir
- 刊名：Journal of Clinical Virology
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：74-81
- 全文大小：1456 K
文摘
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CMV mutations emergence and persistence must be followed in transplanted patients.
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UL97 L595S mutation is one of the more frequent mutations conferring GCV resistance.
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Sanger and next-generation sequencing technologies must be compared for a routine use.
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Compared to Sanger, NGS allowed a deeper discrimination of UL97 L595S evolution.
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Along with entropy, NGS contributed to gain deeply insight into UL97 variability.