Characterization and directed evolution of BliGO, a novel glycine oxidase from Bacillus licheniformis
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- 作者：Kai Zhanga ; Yiming Guoa ; Pei Yaoa ; Yongjun Lina ; Ashok Kumara ; Ziduo Liua ; Gaobing Wua ; b ; wgb@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Lili Zhanga ; c ; zhang63lyly@sina.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Glycine oxidase ; Glyphosate ; Directed evolution ; DNA shuffling
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：85
- 期：Complete
- 页码：12-18
- 全文大小：1557 K
文摘
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A cold-adapted glycine oxidase (BliGO) with glyphosate-degrading activity was cloned from Bacillus licheniformis.
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The catalytic efficiency of BliGO toward glyphosate was increased by 8-fold by in-vitro directed evolution.
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Residue Ser51 might involve in the binding of enzyme with glyphosate in the evolved mutant.