Temporal regulation of gap junctional communication between tenocytes subjected to static tensile strain with physiological and non-physiological amplitudes

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• 作者：Eijiro Maeda^a ; ^{e.maeda@nagoya-u.jp} ; Haicheng Pian^b ; Toshiro Ohashi^a
• 关键词：Gap junction ; GJIC ; Static tensile strain ; Mechanobiology ; Tendon ; Tenocyte
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：1170-1175
• 全文大小：778 K
• 卷排序：482

文摘

Temporal regulation of gap junctional intercellular communication (GJIC) between isolated tenocytes under static tensile strain were investigated. Connexin 43 (Cx43) gene expression as well as Cx43 protein localisations were also examined. The application of 4% static strain induced GJIC at 1 h, which was maintained for several hours. The application of 8% static strain inhibited GJIC at 1 h, which was also maintained for several hours. Cx43 gene expression was transiently upregulated at 1 h with the 4% strain, while that was continuously downregulated with the 8% strain. The findings obtained highlights the differences in mechanical regulation of GJIC under the physiological (4%) and non-physiological (8%) strain amplitudes.