High-resolution, preparative purification of PEGylated protein using a laterally-fed membrane chromatography device
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- 作者：Pedram Madadkar ; Sergio Luna Nino ; Raja Ghosh ; rghosh@mcmaster.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Membrane chromatography ; Device ; PEGylated protein ; Laterally-fed membrane chromatography ; Protein purification
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 November 2016
- 年：2016
- 卷：1035
- 期：Complete
- 页码：1-7
- 全文大小：1337 K
文摘
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Single-step membrane chromatography purification of mono-PEGylated protein.
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Combination of high-resolution with high-productivity.
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Resolution comparable to resin bed column.
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Significantly faster than resin bed column.
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Greater number of theoretical plates per meter than equivalent column.
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Separation in less bed volumes than equivalent column.