Development of a single-nucleotide-polymorphism marker for specific authentication of Korean ginseng (Panax ginseng Meyer) new cultivar “G-1”

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• 作者：Dong-Uk Yang¹ ; ^☆ ; Min-Kyeoung Kim² ; ^☆ ; ^{kyeoung0207@khu.ac.kr} ; Padmanaban Mohanan³ ; Ramya Mathiyalagan³ ; Kwang-Hoon Seo¹ ; Woo-Saeng Kwon¹ ; Deok-Chun Yang¹ ; ³ ; ^{dcyang@khu.ac.kr}
• 关键词：G-1 cultivar ; multiplex polymerase chain reaction ; Panax ginseng ; Panax quinquefolius ; Single-nucleotide polymorphism
• 刊名：Journal of Ginseng Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：41
• 期：1
• 页码：31-35
• 全文大小：1221 K
• 卷排序：41

文摘

Korean ginseng (Panax ginseng) is a well-known medicinal plant of Oriental medicine that is still in practice today. Until now, a total of 11 Korean ginseng cultivars with unique features to Korean ginseng have been developed based on the pure-line-selection method. Among them, a new cultivar namely G-1 with different agricultural traits related to yield and content of ginsenosides, was developed in 2012.MethodsThe aim of this study was to distinguish the new ginseng cultivar G-1 by identifying the unique single-nucleotide polymorphism (SNP) at its 45S ribosomal DNA and Panax quinquefolius region than other Korean ginseng cultivars using multiplex amplification-refractory mutation system–polymerase chain reaction (ARMS-PCR).ResultsA SNP at position of 45S ribosomal DNA region between G-1, P. quinquefolius, and the other Korean ginseng cultivars was identified. By designing modified allele-specific primers based on this site, we could specifically identified G-1 and P. quinquefolius via multiplex PCR. The unique primer for the SNP yielded an amplicon of size 449 bp in G-1 cultivar and P. quinquefolius. This study presents an effective method for the genetic identification of the G-1 cultivar and P. quinquefolius.ConclusionThe results from our study shows that this SNP-based approach to identify the G-1 cultivar will be a good way to distinguish accurately the G-1 cultivar and P. quinquefolius from other Korean ginseng cultivars using a SNP at 45S ribosomal DNA region.