Chemoenzymatically prepared konjac ceramide inhibits NGF-induced neurite outgrowth by a semaphorin 3A-like action
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- 作者：Seigo Usukia ; susuki@sci.hokudai.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Noriko Tamurab ; Shota Sakaia ; Tomohiro Tamurab ; Katsuyuki Mukaic ; Yasuyuki Igarashia
- 关键词：NGF ; nerve growth factor ; Cer ; ceramide ; GlcCer ; glucosylceramide ; kCer ; konjac ceramide ; C2Cer ; N-acetyl-D-erythro-sphingosine ; C16Cer ; N-hexadecanoyl-D-erythro-sphingosine ; C18Cer ; N-octadecanoyl-D-erythro-sphingosine ; C24Cer ; N-tetracosanoyl-D-erythro-sphingosine ; EGCase I ; endoglycoceramidase I ; CRMP2 ; collapsin response mediator protein 2 ; pCRMP2 ; phospho-collapsin response mediator protein 2 ; LDH ; lactate dehydrogenase ; TBEA ; trypan blue exclusion assay ; CCK-8 ; cell counting kit 8 ; Sema 3A
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：5
- 期：Complete
- 页码：160-167
- 全文大小：5566 K
文摘
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We prepared konjac ceramide (kCer) from konjac GlcCer using endoglycoceramidase I.
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kCer inhibits NGF-induced neurite outgrowth by semaphorin 3A-like action.
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Konjac GlcCer does not show NGF-induced neurite outgrowth such as kCer.