Purification and characterization of a glycosidase with hydrolyzing multi-3-O-glycosides of spirostanol saponin activity from Gibberella intermedia
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- 作者：Hui Li ; Xiangdong Wang ; Yang Ma ; Nannan Yang ; Xiaojuan Zhang ; Zhenghong Xu ; Jinsong Shi ; shijs@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Purification ; Glycosidase ; Diosgenin ; Substrate specificity ; Gibberella intermedia WX12
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：46-51
- 全文大小：912 K
文摘
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Gibberella intermedia had ability to hydrolyze dioscin from DZW into diosgenin.
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A special glycosidase from G. intermedia was purified and characterized.
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The glycosidase showed the maximum activity at 50 °C and pH 8.0.
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The glycosidase was stable in the pH range of 5.0–8.0.
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The glycosidase exhibited high activity on dioscin, trillin, and polyphyllin VII.