Preparation of poly(vinyltetrazole) chain-grafted poly(glycidymethacrylate-co-ethylenedimethacrylate) beads by surface-initiated atom transfer radical polymerization for the use in weak cation exchange and hydrophilic interaction chromatography

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• 作者：Junxia Hao^a ; Fuqiang Wang^a ; Xiaojun Dai^a ; Bolin Gong^a ; ^{gongbl@nxu.edu.cn"" rel=""nofollow} ; ^{gongbolin@163.com"" rel=""nofollow} ; Yinmao Wei^b ; ^{ymwei@nwu.edu.cn"" rel=""nofollow}
• 关键词：Surface-initiated atom transfer radical polymerization ; Weak cation exchange chromatography ; Hydrophilic interaction chromatography ; Polyvinyltetrazole ; Proteins ; Nucleosides
• 刊名：Talanta
• 出版年：2011
• 出版时间：15 July 2011
• 年：2011
• 卷：85
• 期：1
• 页码：482-487
• 全文大小：568 K

文摘

A novel stationary phase for weak cation exchange (WCX) and hydrophilic interaction chromatography (HILIC) was prepared with surface-initiated atom transfer radical polymerization (SI-ATRP). Vinyltetrazole was grafted onto the surface of the beads in water medium with the polyglycidylmethacrylate beads (P_GMA/EDMA) previously modified with 2-bromoisobutryl bromide as the macromolecule initiators and CuCl as catalyst. The poly(vinyltetrazole)-grafted beads obtained with different atom transfer radical polymerization (ATRP) formulations were tried as chromatographic packings in ion-exchange chromatography. The results showed that the prepared columns could separate the tested proteins with high efficiency and high capacity, and the retention time of protein had a positive relationship with increasing the chain lengths of the grafted poly(vinyltetrazole) (PVT). The prepared column was also found to be able to separate nucleosides by hydrophilic interaction chromatographic mode.