Early responses of mature Arabidopsis thaliana plants to reduced water potential in the agar-based polyethylene glycol infusion drought model

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• 作者：Andrej Frolov^a ; ^b ; ¹ ; ^{afrolov@ipb-halle.de} ; Tatiana Bilova^a ; ^b ; ^c ; ¹ ; Gagan Paudel^a ; ^b ; Robert" data-ln="Berger" data-pos="4" data-tb="--> ; Robert Berger^a ; Gerd U." data-ln="Balcke" data-pos="5" data-tb="--> ; Gerd U. Balcke^d ; Claudia Birkemeyer^b ; Ludger A." data-ln="Wessjohann" data-pos="7" data-tb="--> ; Ludger A. Wessjohann^a
• 关键词：ABA ; abscisic acid ; Asc ; ascorbic acid ; BHT ; butylated hydroxytoluene ; CID ; collision induced dissociation ; DDA ; data-dependent acquisition ; DHA ; dehydroascorbate ; DTT ; dithiothreitol ; ESI ; electrospray ionization ; FOX ; ferrous ion oxidation xylenol orange ; GC ; gas chromatography ; GSH ; reduced glutathione ; GSSG ; oxidized glutathione ; ID ; internal diameter ; LC ; liquid chromatography ; LEA ; late-embryogenesis-abundant ; LipOOH ; lipid hydroperoxides ; LIT ; linear ion trap ; MES ; 2-(N-morpholino)ethanes
• 刊名：Journal of Plant Physiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：208
• 期：Complete
• 页码：70-83
• 全文大小：1826 K
• 卷排序：208

文摘

Drought is one of the most important environmental stressors resulting in increasing losses of crop plant productivity all over the world. Therefore, development of new approaches to increase the stress tolerance of crop plants is strongly desired. This requires precise and adequate modeling of drought stress. As this type of stress manifests itself as a steady decrease in the substrate water potential (ψw), agar plates infused with polyethylene glycol (PEG) are the perfect experimental tool: they are easy in preparation and provide a constantly reduced ψw, which is not possible in soil models. However, currently, this model is applicable only to seedlings and cannot be used for evaluation of stress responses in mature plants, which are obviously the most appropriate objects for drought tolerance research. To overcome this limitation, here we introduce a PEG-based agar infusion model suitable for 6–8-week-old A. thaliana plants, and characterize, to the best of our knowledge for the first time, the early drought stress responses of adult plants grown on PEG-infused agar. We describe essential alterations in the primary metabolome (sugars and related compounds, amino acids and polyamines) accompanied by qualitative and quantitative changes in protein patterns: up to 87 unique stress-related proteins were annotated under drought stress conditions, whereas further 84 proteins showed a change in abundance. The obtained proteome patterns differed slightly from those reported for seedlings and soil-based models.