Identification and validation of reference genes for quantitative RT-PCR analysis of retinal pigment epithelium cells under hypoxia and/or hyperglycemia
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- 作者：Xin Liu emperorsoninlow@163.com" class="auth_mail" title="E-mail the corresponding author ; Jia&rsquo ; nan Xie 1120870016@qq.com" class="auth_mail" title="E-mail the corresponding author ; Zaoxia Liu 55200369@qq.com" class="auth_mail" title="E-mail the corresponding author ; Qiaoyun Gong 592393704@qq.com" class="auth_mail" title="E-mail the corresponding author ; Rui Tian 781863375@qq.com" class="auth_mail" title="E-mail the corresponding author ; Guanfang Su ; sugf2012@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：DR ; diabetic retinopathy ; RPE ; retinal pigment epithelium ; HIF-1α ; hypoxia induced factor-1α ; VEGF ; vascular endothelial growth factor ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; Rn18s ; 18S ribosomal RNA ; ACTB ; actin ; beta ; ALAS1 ; 5&prime ; -aminolevulinate synthase 1 ; B2M ; beta-2-microglobulin ; GUSB ; glucuronidase ; beta ; HPRT1 ; hypoxanthine phosphoribosyl transferase 1 ; HMBS ; hydroxymethylbilane synthase ; PPIA ; peptidyl prolylisomerase A ; PUM1 ; pumilio RNA binding family member 1 ; RPL29 ; ri
- 刊名：Gene
- 出版年：2016
- 出版时间：10 April 2016
- 年：2016
- 卷：580
- 期：1
- 页码：41-46
- 全文大小：685 K
文摘
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HPRT1, GUSB and PPIA are the most suitable reference genes for RPE cell gene expression study with hypoxia and/or hyperglycemia.
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The commonly used GAPDH and ACTB were ranked only in the middle place and referred unstable among all the candidate genes.
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Importance of choosing proper reference gene was confirmed by comparing HIF-1α expression normalized to varying reference genes.