The conformational change of rabbit muscle pyruvate kinase induced by activating cations and its substrates

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• 作者：Yan Ou ; Wenbing Tao ; Yun Zhang ; Guangrong Wu ; Shaoning Yu
• 关键词：Pyruvate kinase ; Fluorescence ; Acrylamide quenching ; Conformational change
• 刊名：International Journal of Biological Macromolecules
• 出版年：2010
• 出版时间：1 August 2010
• 年：2010
• 卷：47
• 期：2
• 页码：228-232
• 全文大小：294 K

文摘

Catalysis by rabbit muscle pyruvate kinase involves domain movements and conformational changes induced by activating cations and its substrates. Fluorescence acrylamide quenching analyses reveal that interactions with Mg²⁺ and K⁺ lead to a more exposed active site of PK while interactions with PEP and ADP decrease solvent accessibility of the active site.