Identification of a marine NADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes

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• 作者：Guangyue Li ; Jie Ren ; Qiaqing Wu ; ^{wu_qq@tib.cas.cn} ; Jinhui Feng ; Dunming Zhu ; ^{zhu_dm@tib.cas.cn} ; Yanhe Ma
• 关键词：Oceanospirillum sp. ; Aldehyde reductase ; Bioreduction ; Chemoselectivity ; Marine enzyme
• 刊名：Journal of Molecular Catalysis B: Enzymatic
• 出版年：2013
• 出版时间：June, 2013
• 年：2013
• 卷：90
• 期：Complete
• 页码：17-22
• 全文大小：1598 K

文摘

A putative aldehyde reductase gene from Oceanospirillum sp. MED92 was overexpressed in Escherichia coli. The recombinant protein (OsAR) was characterized as a monomeric NADPH-dependent aldehyde reductase. The kinetic parameters K_m and k_cat of OsAR were 0.89 ¡À 0.08 mM and 11.07 ¡À 0.99 s^?1 for benzaldehyde, 0.04 ¡À 0.01 mM and 6.05 ¡À 1.56 s^?1 for NADPH, respectively. This enzyme exhibited high activity toward a variety of aromatic and aliphatic aldehydes, but no activity toward ketones. As such, it catalyzed the chemoselective reduction of aldehydes in the presence of ketones, as demonstrated by the reduction of 4-acetylbenzaldehyde or the mixture of hexanal and 2-nonanone, showing the application potential of this marine enzyme in such selective reduction of synthetic importance.