The midbrain slice preparation. An in vitro model to select potential anti-parkinsonian drugs?

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• 作者：Ezia Guatteo ; Maria Letizia Cucchiaroni ; Luca Sebastianelli ; Giorgio Bernardi ; Nicola Biagio Mercuri
• 关键词：Parkinson's disease ; Electrophysiology ; Dopamine agonists ; In vitro model
• 刊名：Parkinsonism and Related Disorders
• 出版年：2008
• 出版时间：July 2008
• 年：2008
• 卷：14
• 期：Supplement 2
• 页码：S150-S153
• 全文大小：154 K

文摘

Most anti-parkinsonian drugs produce a dopamine-like effect on single dopaminergic neurons. Using electrophysiological recordings, this effect is observed as an inhibition of the spontaneous firing activity. The firing activity of dopaminergic cells throughout dopamine-mediated mechanisms is inhibited not only by dopamine direct agonists but also by substances that increase the release or synthesis, or block the uptake or reduce degradation of dopamine in the brain. Here we propose the electrophysiological approach on dopaminergic neurons in in vitro slice preparation as a preliminary tool for selecting new potential anti-parkinsonian agents, before they are tested in more complex animal models.