Trypanosoma cruzi

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• 作者：Eliane R.P. Lala ; Miriam H. Andó ; Leila Zalloum ; Marta Bé ; rtoli ; Má ; rcia de Oliveira Machado Dalalio ; Thais Gomes Verzignassi Silveira ; Mô ; nica L. Gomes ; Terezinha A. Guedes ; Silvana
• 关键词：Trypanosoma cruzi ; Genetics– ; biology correlation ; Mantel’ ; s test ; Group analysis ; Principal components analysis ; Cladistical analysis
• 刊名：Experimental Parasitology
• 出版年：2009
• 出版时间：October 2009
• 年：2009
• 卷：123
• 期：2
• 页码：173-181
• 全文大小：619 K

文摘

The correlation of genetic and biological diversity in Trypanosoma cruzi was studied. Strains of T. cruzi II, isolated from humans; and of T. cruzi I, isolated from wild-animal reservoirs and from triatomines in the state of Paraná, Brazil, were used. Thirty-six biological parameters measured in vitro and six in vivo, related to growth kinetics and metacyclogenesis, susceptibility to benznidazole, macrophage infection, and experimental infection in mice were evaluated. Data from RAPD and SSR-PCR were used as genetic parameters. Mantel’s test, group analysis, principal components analysis (PCA), and cladistical analyses were applied. With the Mantel’s test, a low correlation was observed when parameters related to growth kinetics and metacyclogenesis in vitro and development of the experimental infection in vivo were included. The group analysis defined two groups that were separated as to whether they produced patent parasitemia in BALB/c mice. In the larger group, strains derived from wild reservoirs were separated from strains derived from triatomines and humans. The PCA identified two groups that differed as to whether they produced a parasitemia curve in mice. The cladistical analysis supported the previous results. This study shows the importance of the parasite–host relationship for the behavior of the strains, and that the combination of methods supports, extends, and clarifies the available information.