- 作者：Aihua Gu ; Guixiang Ji ; Pengfei Zhu ; Yong Zhou ; Guangbo Fu ; Yankai Xia ; Ling Song ; Shoulin Wang ; Xinru Wang
- 关键词：Polycyclic aromatic hydrocarbons (PAHs) ; nucleotide excision repair (NER) ; xeroderma pigmentosum group A gene (XPA) ; polymorphism ; gene-environment interaction ; sperm DNA damage
- 刊名：Fertility and Sterility
- 出版年：2010
- 出版时间：December 2010
- 年：2010
- 卷：94
- 期：7
- 页码：2620-2625.e5
- 全文大小：490 K
Objective
To evaluate the effects of polycyclic aromatic hydrocarbon (PAH) exposure and four functional genetic polymorphisms in the nucleotide excision repair pathway, alone or combined, on sperm DNA integrity and male fertility.Design
Retrospective case-only and case-control study.
Setting
One university and three centers of clinical reproductive medicine in the Jiangsu Province of China.
Patient(s)
Six hundred twenty infertile men and 273 controls were recruited for the study.
Intervention(s)
None.
Main Outcome Measure(s)
Exposure to PAHs was detected as urinary 1-hydroxypyrene level. Genotypes were determined by polymerase chain reaction–restriction fragment length polymorphism, and sperm DNA damage was detected by terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling assay with use of flow cytometry.
Result(s)
Increased sperm DNA damage was found to be associated significantly with increased urinary concentrations of 1-hydroxypyrene. We also observed that the xeroderma pigmentosum group A (XPA)-4 G/A polymorphism was associated with sperm DNA damage. Subjects homozygous for XPA-4 AA had significantly more sperm DNA damage compared with subjects for XPA-4 GG. Moreover, on the basis of a case-control study, a significant interaction was found between XPA-4 G/A polymorphism and PAH exposures on the sperm DNA damage risk (odds ratio = 2.86, 95 % confidence interval = 1.77–4.61).
Conclusion(s)
We provide the first evidence for potential gene-environment interactions between nucleotide excision repair polymorphisms and PAH exposure on the sperm DNA damage and male factor infertility in men with no occupational exposure to PAHs.