Tumor necrosis factor alpha gene variants do not display allelic imbalance in circulating myeloid cells

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• 作者：S ; ra Wienzek ; Karin Kissel ; Kirstin Breithaupt ; Christina Lang ; Angelika Nockher ; Holger Hackstein ; Gregor Bein
• 关键词：Tumor necrosis factor α ; Genetic polymorphism ; Risk allele ; TNF − ; 308 A/G ; Allelic imbalance
• 刊名：Cellular Immunology
• 出版年：2010
• 出版时间：2010
• 年：2010
• 卷：262
• 期：2
• 页码：127-133
• 全文大小：425 K

文摘

Carriage of the TNF −308 A allele (rs1800629 A) has been associated with increased serum TNF-α levels, the development of sepsis syndrome, and fatal outcome, in severely traumatized patients (Menges et al., 2008 [1]). Herein, we analysed the putative allelic imbalance of TNF-α release from myeloid cells. Circulating peripheral blood cells from healthy human blood donors (n = 104) and monocyte-derived macrophages (n = 158) were analysed for their ex vivo capacity of TNF-α expression. Our findings indicate that carriage of the TNF −308 A allele is not associated with high TNF-α expression in circulating human leucocytes and monocyte-derived macrophages. Other cellular sources, e.g. tissue-resident cells like mast cells and/or tissue specific macrophages might be the cellular source of high TNF-α serum levels shortly after trauma.