A bicyclo-hairpin probe mediated strand displacement amplification strategy for label-free and sensitive detection of bleomycin
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- 作者：Huijuan WangaAuthor Vitae ; Wei JiangbAuthor Vitae ; Wei LiaAuthor Vitae ; Lei Wanga ; wangl-sdu@sdu.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Bicyclo-hairpin probe ; Strand displacement amplification ; Label-free ; Bleomycin
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：238
- 期：Complete
- 页码：318-324
- 全文大小：1389 K
文摘
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A bicyclo-hairpin probe was designed ingeniously for bleomycin (BLM) recognition.
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Based on BLM-induced DNA scission, a label-free fluorescent sensor for BLM detection was proposed.
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The bicyclo-hairpin probe could silence trigger sequence to reduce non-specific hybridization.
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The strategy showed a great potential application ability in clinical sample analysis.
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