Improving ante mortem diagnosis of Erysipelothrix rhusiopathiae infection by use of oral fluids for bacterial, nucleic acid, and antibody detection
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- 作者：Luis G. Gimé ; nez-Lirola ; Chao-Ting Xiao ; Marissa Zavala ; Patrick G. Halbur ; T. Opriessnig
- 关键词：Erysipelothrix rhusiopathiae ; Oral fluid ; Diagnostic algorithm ; Pigs
- 刊名：Journal of Microbiological Methods
- 出版年：2013
- 出版时间：15 February, 2013
- 年：2013
- 卷：92
- 期：2
- 页码：113-121
- 全文大小：1042 K
文摘
Swine erysipelas is an economically important disease caused by Erysipelothrix rhusiopathiae. Pen-based collection of oral fluids has recently been utilized for monitoring infection dynamics in swine operations. The diagnostic performance of bacterial isolation, real-time PCR, and antibody detection by enzyme-linked immunosorbent assay (ELISA) and fluorescent microbead-based immunoassay (FMIA) methods were evaluated on pen-based oral fluid samples from pigs experimentally infected with E. rhusiopathiae (n = 112) and from negative controls (n = 32). While real-time PCR was a sensitive method with an overall detection rate of 100 % (7/7 pens) one day post inoculation (dpi), E. rhusiopathiae was successfully isolated in only 28.6 % (2/7 pens). Anti-Erysipelothrix IgM and IgG antibodies in pen-based oral fluids were detected at 4 to 5 dpi by FMIA and at 5 and 8 dpi by ELISA. The number of infected animals per pen, and in particular the timing of antimicrobial treatment administration impacted bacterial isolation and ELISA results. In oral fluid field samples, E. rhusiopathiae DNA was found in 23.3 % of the samples while anti-E. rhusiopathiae IgG and IgM antibodies were found in 59.6 % and 5.5 % of the samples, respectively. The results suggest that an algorithm integrating oral fluids as specimen and real-time PCR and FMIA as detection methods is effective for earlier detection of an erysipelas outbreak thereby allowing for a more effective treatment outcome.