Binding of recombinant T cell receptor ligands (RTL) to antigen presenting cells prevents upregulation of CD11b and inhibits T cell activation and transfer of experimental autoimmune encephalomyelitis

详细信息    查看全文

• 作者：Sushmita Sinha ; Lisa Miller ; S ; hya Subramanian ; Owen J.T. McCarty ; Thomas Proctor ; Roberto Meza-Romero ; Jianya Huan ; Gregory G. Burrows ; Arthur A. V ; enbark ; Halina Offner
• 关键词：EAE ; RTLs ; Tolerance ; APCs
• 刊名：Journal of Neuroimmunology
• 出版年：2010
• 出版时间：25 August 2010
• 年：2010
• 卷：225
• 期：1-2
• 页码：52-61
• 全文大小：1449 K

文摘

Recombinant T cell ligands (RTLs) ameliorate experimental autoimmune encephalomyelitis (EAE) in an antigen-specific manner. We evaluated effects of RTL401 (I-A^s α1β1 + PLP-139-151) on splenocytes from SJL/J mice with EAE to study RTL-T cell tolerance-inducing mechanisms. RTLs bound to B, macrophages and DCs, through RTL-MHC-α1β1 moiety. RTL binding reduced CD11b expression on splenic macrophages/DC, and RTL401-conditioned macrophages/DC, not B cells, inhibited T cell activation. Reduced ability of RTL- incubated splenocytes to transfer EAE was likely mediated through macrophages/DC, since B cells were unnecessary for RTL treatment of EAE. These results demonstrate a novel pathway of T cell regulation by RTL-bound APCs.