Effects of different artificial insemination techniques and sperm doses on fertility of normal mares and mares with abnormal reproductive history

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• 作者：Sieme ; H. ; Bonk ; A. ; Hamann ; H. ; Klug ; E. ; Katila ; T.
• 关键词：Low-dose insemination ; Frozen semen ; Fresh semen ; Hysteroscopic insemination ; Pregnancy rate
• 刊名：Theriogenology
• 出版年：2004
• 出版时间：September 1, 2004
• 年：2004
• 卷：62
• 期：5
• 页码：915-928
• 全文大小：139 K

文摘

The effects of different artificial insemination (AI) techniques and sperm doses on pregnancy rates of normal Hanoverian breed mares and mares with a history of barrenness or pregnancy failure using fresh or frozen–thawed sperm were investigated. The material included 187 normal mares (148 foaling and 39 young maiden mares) and 85 problem mares with abnormal reproductive history. Mares were randomly allotted into groups with respect to AI technique (routine AI into the uterine body, transrectally controlled deep intracornual AI ipsilateral to the preovulatory follicle, or hysteroscopic AI onto the uterotubal junction ipsilateral to the preovulatory follicle), storage method of semen (fresh, frozen–thawed), AI volume (0.5, 2, 12ml), and sperm dose (50×10⁶ or 300×10⁶ progressively motile sperm (pms) for fresh semen and 100 or 800×10⁶ frozen–thawed sperm with >35 % post-thaw motility). The mares were inseminated once per cycle, 24h after hCG administration when fresh semen was used, or 30h for frozen–thawed semen. Differences in pregnancy rates between treatment groups were analyzed by Chi-squared test, and for most relevant factors (insemination technique, mare, semen, and stallion) expectation values and confidence intervals were calculated using multivariate logistic models.