The production of biogas from agricultural resources as an energy source for electrical energy may substantially contribute to the mitigation of GHG emissions by offsetting emissions from fossil resources and by reducing emissions from the storage of animal manure. In the scenarios analyzed GHG emissions calculated with default values were between 0.10 and 0.40 kg CO2-eq/kWhel, which is 22 % –75 % less than the GHG emissions caused by the present energy mix in Germany. The analysis demonstrates the variability of the mitigation effect due to uncertainties with technical and environmental processes, which are difficult to control. Uncertainties due to fertilizer induced N2O emissions from the soil had the biggest impact on the mitigation effect of biogas use when the digestate is stored gas-tight. Otherwise, the uncertainty of emissions from the digestate dominates the variability of GHG emissions of the whole process. Moderate effects are caused by the biogas yield from feedstock, methane leakage, the electrical efficiency of the combined heat and power unit (CHP), and nitrate leaching. A minor impact can be expected from fertilizer volatilization and from the power consumption of the biogas plant.