An electrochemical biosensor for the rapid detection of DNA damage induced by xanthine oxidase-catalyzed Fenton reaction

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• 作者：Huayu XiongAuthor Vitae ; Yang ChenAuthor Vitae ; Xiuhua ZhangAuthor Vitae ; Haoshuang GuAuthor Vitae ; Shengfu Wang ; ^{wangsf@hubu.edu.cn}Author Vitae
• 关键词：XOD ; xanthine oxidase ; OH ; hydroxyl radical ; ROS ; reactive oxygen species ; XA ; xanthine ; DNA&ndash ; XOD/GCE ; dsDNA and a XOD modified glassy carbon electrode ; Co(bpy)3(ClO4)3 ; Tris(2 ; 2&prime ; -bipyridyl) cobalt(III) perchlorate ; AA ; ascorbic acid ; AE ; aloe-
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：181
• 期：Complete
• 页码：85-91
• 全文大小：896 K

文摘

Oxidative DNA damage is one of the most critical factors implicated in carcinogenesis and other disorders. Sensitive and reliable detection of oxidative DNA damage remains a significant challenge. In this work, a sensitive electrochemical biosensor based on a double stranded DNA immobilized on a xanthine oxidase (XOD)-modified glassy carbon electrode (denoted as DNA-XOD/GCE) has been developed to explore the rapid detection of DNA damage. Co(bpy)₃³⁺ was used as a redox indicator to monitor DNA damage induced by hydroxyl radical (OH), which is a reactive oxygen species generated by a XOD-catalyzed Fenton reaction in xanthine/FeSO₄ system. The produced OH was validated by UV-vis spectroscopy. The electrochemical behavior of the underlying electrodes was characterized by square wave voltammetry and electrochemical impedance spectroscopy. Optimization of the concentrations of FeSO₄ and XA, and the incubation time in terms of DNA damage was explored. Moreover, the protection of DNA from damage by antioxidants, such as ascorbic acid, aloe-emodin, and rutin was investigated. The conclusions demonstrate that the proposed electrochemical method is expected to be of use in further application for DNA damage studies.