Effect of hydroalcoholic leaves extract of Citrullus colocynthis on induction of insulin secretion from isolated rat islets of Langerhans

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• 作者：Esmaeel Ebrahimi^a ; Somaieh Bahramzadeh^b ; Mahmoud Hashemitabar^b ; Ghorban Mohammadzadeh^c ; Saeed Shirali^d ; ^{saeed.shirali@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Javad Jodat^a
• 关键词：Diabetes mellitus ; Insulin ; Citrullus colocynthis ; Flavones ; Flavonoids
• 刊名：Asian Pacific Journal of Tropical Disease
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：6
• 期：8
• 页码：638-641
• 全文大小：377 K

文摘

To study the effect of hydroalcoholic leaves extract of Citrullus colocynthis (C. colocynthis) on insulin secretion in isolated Langerhans islets of rat.

Methods

The islets were isolated from male adult Wistar rats' pancreas by enzymatic digestion method. After isolation, the islets were incubated in different doses of hydroalcoholic leaves extract of C. colocynthis (0.01, 0.10 and 1.00 mg/mL Hanks' balanced salt solution for 30 min and then calculated insulin level by ELISA method.

Results

The result showed that C. colocynthis has a potential effect on insulin secretion. It was found that dose of 0.1 mg/mL Hanks' balanced salt solution extract has best affect on induction of insulin secretion.

Conclusions

It is suggested that further studies should be done on insulin releasing effect of secondary metabolites (flavones and flavonoids) isolated from C. colocynthis.