Prevalidation trial for a novel in vitro eye irritation test using the reconstructed human cornea-like epithelial model, MCTT HCE™

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• 作者：Hyeri Yang^a ; Da-eun Kim^a ; Won-Hee Jang^b ; Susun An^b ; Sun-A Cho^b ; Mi-Sook Jung^c ; Ji Eun Lee^c ; Kyung-Wook Yeo^d ; Sang Bum Koh^d ; Tae-Cheon Jeong^e ; Mi-Jeong Kang^e ; Young-Jin Chun^f ; Su-Hyon Lee^g ; Kyung-Min Lim^a ; ^{kmlim@ewha.ac.kr} ; SeungJin Bae^a ; ^{sjbae@ewha.ac.kr}
• 关键词：EIT ; eye irritation test ; RhCE ; reconstructed human cornea-like epithelium ; OECD TG ; OECD Test guidelines ; GHS ; global harmonization system ; WL/BL ; within-/between-laboratory ; ROC ; receiver operating characteristics ; SD ; standard deviation ; AUC ; area under curve ; ICC ; intra-class correlation ; PS ; performance standard
• 刊名：Toxicology in Vitro
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：58-67
• 全文大小：1273 K
• 卷排序：39

文摘

MCTT™ HCE EIT employing water-soluble formazan WST-1, demonstrated a performance comparable to other RhCEs. Within- and between-lab reproducibility for 3 labs with 20 chemicals were 85–95% and 95% respectively. Predictive capacity for 126 chemicals was sensitivity 82.2%, specificity 81.1% and accuracy 81.8%