Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation
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- 作者：Erik McShane1 ; Celine Sin2 ; Henrik Zauber1 ; Jonathan N. Wells3 ; Neysan Donnelly4 ; Xi Wang1 ; Jingyi Hou1 ; Wei Chen1 ; 7 ; Zuzana Storchova4 ; 5 ; Joseph A. Marsh3 ; Angelo Valleriani2 ; Matthias Selbach1 ; 6 ; 8 ; matthias.selbach@mdc-berlin.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：proteomics ; protein degradation ; protein complex assembly ; aneuploidy ; gene copy-number alterations ; posttranslational control ; trisomy ; metabolic labeling ; bioorthogonal amino acid tagging ; pulse chase experiment
- 刊名：Cell
- 出版年：2016
- 出版时间：20 October 2016
- 年：2016
- 卷：167
- 期：3
- 页码：803-815.e21
- 全文大小：13541 K
文摘
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Global pulse-chase experiments identify non-exponentially degraded (NED) proteins
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NED proteins become more stable with age
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Many NED proteins are over-synthesized subunits of multiprotein complexes
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Decay profiles can predict steady-state protein levels in aneuploid cells