Genetic characterization of an adapted pandemic 2009 H1N1 influenza virus that reveals improved replication rates in human lung epithelial cells
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- 作者：Xenia Wö ; rmanna ; 1 ; Markus Lescha ; b ; 1 ; Robert-William Welkec ; Konstantin Okonechnikova ; Mirshat Abdurishida ; Christian Siebenc ; 2 ; Andreas Geissnerd ; e ; Volker Brinkmanna ; Markus Kastnerf ; Andreas Karnerg ; Rong Zhuf ; Peter Hinterdorferf ; Chakkumkal Anishd ; 3 ; Peter H. Seebergerd ; e ; Andreas Herrmannc ; Thomas F. Meyera ; b ; meyer@mpiib-berlin.mpg.de" class="auth_mail" title="E-mail the corresponding author ; Alexander Karlasa ; b ; karlas@mpiib-berlin.mpg.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：A/Hamburg/04/2009 ; Hemagglutinin ; High-throughput sequencing ; Reverse genetics ; Viral adaptation
- 刊名：Virology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：492
- 期：Complete
- 页码：118-129
- 全文大小：3906 K
文摘
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We observed a spontaneous mutation of a 2009-pandemic H1N1 influenza virus in vitro.
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The adaptation led to a 100-fold rise in replication rate in human A549 cells.
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Adaptation was caused by two mutations in the HA gene segment.
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Adaptation correlates with increased fusion pH and decreased receptor affinity.