Genetic manipulation of the bifunctional gene, carRA, to enhance lycopene content in Blakeslea trispora

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• 作者：Yanlong Wang^a ; ^b ; Jing Pang^b ; Yingmei Zheng^b ; Pingping Jiang^b ; Wenfang Gong^b ; Xiwen Chen^c ; ^{xiwenchen@nankai.edu.cn} ; Defu Chen^a ; ^b ; ^{chendefu@nankai.edu.cn}
• 关键词：CarA ; phytoene synthase ; CarB ; phytoene dehydrogenase ; carG ; geranylgeranyl pyrophosphate synthase gene ; CarR ; lycopene cyclase ; coq2 ; 4-hydroxybenzoate polyprenyl transferase gene ; CTAB ; cetyl trimethyl ammonium bromide ; DMAPP ; dimethylallyl pyrophosphate ; erg9 ; ergosterol synthase gene ; FPP ; farnesyl pyrophosphate ; GGPP ; geranylgeranyl pyrophosphate ; HMG-CoA ; 3-hydroxy-3-methylglutaryl-coenzyme A ; HPLC ; high-performance liquid chromatography ; hmgr ; 3-hydroxy-3-methylglutaryl-coenzyme A reducta
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 March 2017
• 年：2017
• 卷：119
• 期：Complete
• 页码：27-33
• 全文大小：922 K
• 卷排序：119

文摘

Manipulation of carRA in B. trispora was conducted using gene targeting technology. Disruption of carR enhanced the lycopene but decreased total carotenoid content. Overexpression of carA increased the lycopene and the total carotenoid content. The target of tripropylamine is the R domain, rather than the A domain, of CarRA.