Anti-HBV activity and mechanism of marine-derived polyguluronate sulfate (PGS) in vitro
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- 作者：Lijuan Wua ; wljwulijuan@126.com" class="auth_mail" title="E-mail the corresponding author ; Wei Wanga ; b ; wwwakin@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xiaoshuang Zhanga ; xiaoshuangzhang89@126.com" class="auth_mail" title="E-mail the corresponding author ; Xia Zhaoa ; b ; zhaoxia@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Guangli Yua ; b ; glyu@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：3TC ; lamivudine ; CC50 ; concentration of 50% cytotoxicity ; DP ; degree of polymerization ; ELISA ; enzyme-linked immunosorbent assay ; FCM ; flow cytometry ; HBeAg ; HBV e antigen ; HBsAg ; HBV surface antigen ; HBV ; hepatitis B virus ; HNF4α ; hepatocyte nuclear factor 4α ; IFN-β ; interferon beta ; MAPK ; mitogen-activated protein kinases ; MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyl tetrazolium bromide ; NF-κB ; nuclear factor-kappa B ; PGS ; Polyguluronate sulfate
- 刊名：Carbohydrate Polymers
- 出版年：2016
- 出版时间：5 June 2016
- 年：2016
- 卷：143
- 期：Complete
- 页码：139-148
- 全文大小：3856 K
文摘
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Polyguluronate sulfate (PGS) effectively inhibits HBV replication in HepG2.2.15 cells.
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PGS inhibited the expression and secretion of HBsAg and HBeAg with low cytotoxicity.
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PGS can bind and enter into HepG2.2.15 cells to interfere with HBV transcription.
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PGS can enhance the production and secretion of interferon β in HepG2.2.15 cells.
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PGS may upregulate NF-κB and Raf/MEK/ERK pathways to enhance the interferon system.