State transition analysis of spontaneous branch migration of the Holliday junction by photon-based single-molecule fluorescence resonance energy transfer
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- 作者：Kenji Okamoto ; Yasushi Sako ; sako@riken.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Single-molecule measurement ; FRET ; Conformational dynamics ; Variational Bayes ; Hidden Markov model ; Single photon counting
- 刊名：Biophysical Chemistry
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：209
- 期：Complete
- 页码：21-27
- 全文大小：801 K
文摘
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Branch migration of Holliday junction (~ 0.7 nm) was observed by smFRET measurement.
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State transition trajectories were reproduced by photon-based VB-HMM analysis.
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State transitions were resolved at upper limit of time resolution (~ 1000 photons).
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Migration kinetics was specified as single- and multi-base-pair steps with rates.
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Branch migration is suitable as a standard sample for smFRET measurement.