Local and cardiorenal effects of periodontitis in nitric oxide-deficient hypertensive rats

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• 作者：Bruno S. Herrera ; Rodrigo Martins-Porto ; Paula Campi ; Marinella Holzhausen ; Simone A. Teixeira ; Gustavo D. Mendes ; Soraia K.P. Costa ; Robert Gyurko ; Thomas E. Van Dyke ; Luis C. Spolidó ; rio ; Marcel
• 关键词：Periodontitis ; Nitric oxide ; Neutrophils ; Nitrotyrosine ; Lipid peroxidation ; Heart ; Kidney
• 刊名：Archives of Oral Biology
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：56
• 期：1
• 页码：41-47
• 全文大小：382 K

文摘

Objective

In this study we have assessed the renal and cardiac consequences of ligature-induced periodontitis in both normotensive and nitric oxide (NO)-deficient (L-NAME-treated) hypertensive rats.

Materials and methods

Oral L-NAME (or water) treatment was started two weeks prior to induction of periodontitis. Rats were sacrificed 3, 7 or 14 days after ligature placement, and alveolar bone loss was evaluated radiographically. Thiobarbituric reactive species (TBARS; a lipid peroxidation index), protein nitrotyrosine (NT; a marker of protein nitration) and myeloperoxidase activity (MPO; a neutrophil marker) were determined in the heart and kidney.

Results

In NO-deficient hypertensive rats, periodontitis-induced alveolar bone loss was significantly diminished. In addition, periodontitis-induced cardiac NT elevation was completely prevented by L-NAME treatment. On the other hand L-NAME treatment enhanced MPO production in both heart and kidneys of rats with periodontitis. No changes due to periodontitis were observed in cardiac or renal TBARS content.

Conclusions

In addition to mediating alveolar bone loss, NO contributes to systemic effects of periodontitis in the heart and kidney.