Efficient production of an avian adeno-associated virus vector using insect cell/baculovirus expression system

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• 作者：Anping Wang ; Yongjuan Wang ; Shuang Wu ; Weiyong Zuo ; Changming Guo ; Weiming Hong ; Shanyuan Zhu ; ^{wap4017@163.com}
• 关键词：Insect cell/baculovirus expression system ; Recombinant avian adeno-associated virus
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：240
• 期：Complete
• 页码：26-31
• 全文大小：1196 K
• 卷排序：240

文摘

The AAAV proteins can be expressed efficiently in the rBac-infected insect cells. The AAAV particles can be assembled correctly in insect cells without the helper viruses. The AAAV vector produced in insect cells can mediate the expression of GFP in chicken cells. The titer of rAAAV produced in insect cells is greatly improved. A scalable and efficient platform for producing rAAAV has been established.