Femtogram ultrasensitive aptasensor for the detection of OchratoxinA

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• 作者：Wei Ma¹ ; Honghong Yin¹ ; Liguang Xu ; Zhou Xu ; Hua Kuang ; Libing Wang ^{khecho@163.com} ; Chuanlai Xu ; ^{xcl@jiangnan.edu.cn}
• 关键词：OTA detection ; Aptasensor ; Real-time PCR ; Food safety
• 刊名：Biosensors and Bioelectronics
• 出版年：2013
• 出版时间：15 April, 2013
• 年：2013
• 卷：42
• 期：Complete
• 页码：545-549
• 全文大小：430 K

文摘

A simple and ultrasensitive method was developed for the detection of Ochratoxin A, utilizing an aptamer as a molecular recognition probe and real-time quantitative PCR (RT-qPCR) amplification of its complementary DNA as signal generators. Under the optimized conditions, the cycle threshold (Ct) increased linearly with 10-fold serial dilutions of Ochratoxin A (OTA) from 5¡Á10^?6 to 5 ng mL^?1, with a limit of detection (LOD) of 1 fg mL^?1. The specificity of this aptasensor was considered to be excellent, as when tested against four other toxins it produced no obvious Ct value change. Furthermore, a satisfactory analyte concentration recovery was obtained from a series of concentrations of OTA spiked into red wine. Therefore, this highly sensitive approach shows a significant potential in a wide range of target analytes.