Towards interference free HPLC-SERS for the trace analysis of drug metabolites in biological fluids

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• 作者：Waleed A. Hassanain ; Emad L. Izake ; ^{e.kiriakous@qut.edu.au} ; Arumugam Sivanesan ; Godwin A. Ayoko
• 关键词：Paper SERS substrate ; HPLC-SERS ; Biological fluids ; PSI-6206 nucleoside ; Infectious diseases
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：20 March 2017
• 年：2017
• 卷：136
• 期：Complete
• 页码：38-43
• 全文大小：1053 K
• 卷排序：136

文摘

Sofosbuvir metabolite was studied for the first time by Raman spectroscopy. Cheap and disposable paper substrate was utilized for the determination of PSI-6206 by HPLC-SERS. The use of disposable substrate eliminated the memory effect problem in the HPLC-SERS. The SERS LOQ of PSI-6206 was 13 ng L-1 (R2 = 0.959, RSD = 5.23%), 4 orders of magnitude less than HPLC-DAD. The HPLC-SERS method provided unique structural identification of PSI-6206 similar to HPLC-MS