The development of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous quantification of morphine, morphine-3-β-glucuronide, morphine-6-β-glucuronide, hydromorphone, and normorphine in serum
详细信息 查看全文
- 作者：David Sartoria ; Tamorah Lewisb ; Autumn Breauda ; William Clarkea ; wclarke@jhmi.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：HPLC&ndash ; MS/MS ; Neonatal abstinence syndrome ; Morphine ; Glucuronide ; Method validation
- 刊名：Clinical Biochemistry
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：48
- 期：18
- 页码：1283-1290
- 全文大小：627 K
文摘
- •
HPLC/MS/MS method validation for morphine and its metabolites
- •
Method validation followed the new CLSI C62-A guidelines.
- •
Analytical measuring range for all analytes was determined to be 5 to 1000 ng/mL.
- •
Method required small sample volume (50 μL) of serum or plasma.