Effects of an endothelial cell-conditioned medium on the hematopoietic and endothelial differentiation of embryonic stem cells

详细信息    查看全文

• 作者：Xuan Sun ; Lamei Cheng ; Huaxin Duan ; Guangxiu Lu
• 关键词：Conditioned medium ; DiI-Ac-LDL ; Embryonic stem cells ; Endothelial cells ; Hematopoietic cells
• 刊名：Cell Biology International
• 出版年：2009
• 出版时间：November 2009
• 年：2009
• 卷：33
• 期：11
• 页码：1201-1205
• 全文大小：649 K

文摘

We have examined the effect of mouse bone marrow endothelial cell-conditioned medium (mEC-CM) on hematopoietic and endothelial differentiation of mouse embryonic stem cells (mESCs). mEC-CM can efficiently promote the differentiation of mESCs into Flk⁺ cells and hematopoietic colony-forming cells. mEC-CM proved to be as potent as a cytokine cocktail comprised of VEGF, bFGF, IGF and EGF. After inducing mESCs with mEC-CM, cobblestone-like cells were mechanically selected and identified which had the ability to incorporate DiI-Ac-LDL. DiI-Ac-LDL-positive cells were endothelial-like cells due to their expression of CD31 and Flk1, ability to bind to UEA1 and capacity to form capillary-like tube structures on matrigel. In conclusion, mEC-CM can efficiently promote the differentiation of mESCs into endothelial cells and hematopoietic colony-forming cells. The differentiated endothelial-like cells can be isolated by using DiI-Ac-LDL labeling and mechanical selection.