Vermicompost humic acids modulate the accumulation and metabolism of ROS in rice plants

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• 作者：Andr&eacute ; s Calderí ; n Garcí ; ap>ap>p> ; p>p>cg.andres@gmail.com" class="auth_mail" title="E-mail the corresponding authorp>p> ; p>p>calderin@ufrrj.br" class="auth_mail" title="E-mail the corresponding authorp> ; Leandro Azevedo Santosp>ap>p> ; p>p>leoazevedo2001@yahoo.com.br" class="auth_mail" title="E-mail the corresponding authorp> ; Luiz Gilberto Ambró ; sio de Souzap>ap>p> ; p>p>betdemolay@hotmail.com" class="auth_mail" title="E-mail the corresponding authorp> ; Orlando Carlos Huertas Tavaresp>ap>p> ; p>p>ochtavares@gmail.com" class="auth_mail" title="E-mail the corresponding authorp> ; Everaldo Zontap>ap>p> ; p>p>ezonta@ufrrj.br" class="auth_mail" title="E-mail the corresponding authorp> ; Ernane Tarcisio Martins Gomesp>ap>p> ; p>p>ernane_floresta@yahoo.com.br" class="auth_mail" title="E-mail the corresponding authorp> ; Jos&eacute ; Maria Garcí ; a-Minap>bp>p> ; p>p>jgmina@timacagro.es" class="auth_mail" title="E-mail the corresponding authorp> ; Ricardo Luis Louro Berbarap>ap>p> ; p>p>rberbara@hotmail.com" class="auth_mail" title="E-mail the corresponding authorp>
• 关键词：ROS ; reactive oxygen species ; DAB ; 3 ; 3&prime ; -diaminobenzidine ; DAT ; days after transplanting ; DOM ; dissolved organic matter ; DOM&mdash ; HS ; dissolved organic matter&mdash ; humic substances ; HA ; humic acid ; H2O2 ; hydrogen peroxide ; NBT ; p-nitro tetrazolium ; PEG ; polyethylene glycol ; P5CS ; Δ-pyrroline-5-carboxylate synthase ; VCHAs ; vermicompost humic acids
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：15 March 2016
• 年：2016
• 卷：192
• 期：Complete
• 页码：56-63
• 全文大小：1553 K

文摘

This work aims to determine the reactive oxygen species (ROS) accumulation, gene expression, anti-oxidant enzyme activity, and derived effects on membrane lipid peroxidation and certain stress markers (proline and malondialdehyde-MDA) in the roots of unstressed and PEG-stressed rice plants associated with vermicompost humic acid (VCHA) application. The results show that the application of VCHA to the roots of unstressed rice plants caused a slight but significant increase in root ROS accumulation and the gene expression and activity of the major anti-oxidant enzymes (superoxide dismutase and peroxidase). This action did not have negative effects on root development, and an increase in both root growth and root proliferation occurred. However, the root proline and MDA concentrations and the root permeability results indicate the development of a type of mild stress associated with VCHA application. When VCHA was applied to PEG-stressed plants, a clear alleviation of the inhibition in root development linked to PEG-mediated osmotic stress was observed. This was associated with a reduction in root ROS production and anti-oxidant enzymatic activity caused by osmotic stress. This alleviation of stress caused by VCHA was also reflected as a reduction in the PEG-mediated concentration of MDA in the root as well as root permeability. In summary, the beneficial action of VCHA on the root development of unstressed or PEG-stressed rice plants clearly involves the modulation of ROS accumulation in roots.