Systematic identification of hepatitis E virus ORF2 interactome reveals that TMEM134 engages in ORF2-mediated NF-κB pathway

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• 作者：Yabin Tian^a ; ¹ ; ^{tianyabin@nifdc.org.cn} ; Weijin Huang^a ; ¹ ; ^{huangweijin@nifdc.org.cn} ; Jun Yang^b ; ^{Jun.Yang2@stjude.org} ; Zhiheng Wen^a ; ^{zhihengw_bj@126.com} ; Yansheng Geng^c ; ^{ysgeng09@aliyun.com} ; Chenyan Zhao^a ; ^{zhaochenyan@nifdc.org.cn} ; Heqiu Zhang^d ; ^{zhangheqiu2004@126.com} ; Youchun Wang^a ; ^{wangyc@nifdc.org.cn}
• 关键词：Hepatitis E virus ; ORF2 ; Split-ubiquitin yeast two-hybrid screening ; Pull-down ; TMEM134
• 刊名：Virus Research
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：228
• 期：Complete
• 页码：102-108
• 全文大小：1312 K
• 卷排序：228

文摘

59 proteins interacting with HEV ORF2 were identified from a human liver cDNA library using an ubiquitin-split yeast two-hybrid approach. 20 ORF2-interacting proteins were located in endoplasmic reticulum (ER). Eight proteins named ASGR2, BNIP3, C14ORF1, EBP, HMOX2, TMEM134, TMEM14A and TMEM230 were further verified by a pull-down assay. TMEM134 interacts and co-localizes with ORF2 in the endoplasmic reticulum, negatively regulates ORF2-mediated inhibition of the NF-κB signaling pathway.