Development of UV cross-linked gelatin coated electrospun poly(caprolactone) fibrous scaffolds for tissue engineering

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• 作者：T.R. Correia^a ; P. Ferreira^b ; ^{pferreira@eq.uc.pt" class="auth_mail" title="E-mail the corresponding author} ; R. Vaz^b ; P. Alves^b ; M.M. Figueiredo^b ; I.J. Correia^a ; ^{icorreia@fcsaude.ubi.pt" class="auth_mail" title="E-mail the corresponding author} ; P. Coimbra^b
• 关键词：Electrospinning ; photocrosslinking ; scaffolds ; gelatin ; biocompatibility
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：93
• 期：part_PB
• 页码：1539-1548
• 全文大小：4635 K

文摘

Cardiovascular disease is the leading cause of morbidity and mortality among industrialized countries. Vascular grafts are often required for the surgical treatments. Considering the limitations associated with the use of autografts and with the currently available synthetic materials, a growing demand in tissue engineered vascular grafts has been registered.

During the work here described, electrospinning technique was used to prepared fibrous matrices to be applied as vascular implants. For that purpose, electrospun polycaprolactone (PCL) fibrous mats were produced and afterwards coated with different hydrogel formulations based in photocrosslinkable gelatin (GelMA) and the macromers poly(ethylene glycol) acrylate (PEGA) and poly(ethylene glycol) diacrylate (PEGDA). These were further photocrosslinked under UV irradiation using Irgacure^® 2959 (by BASF) as the photoinitiator.

The suitability of the coated scaffolds for the intended application, was evaluated by assessing their chemical/physical properties as well as their interaction with blood and endothelial cells.