Effective asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate by recombinant E. coli CCZU-A13 in [Bmim]PF6-hydrolyzate media
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- 作者：Yu-Cai Hea ; b ; c ; heyucai2001@163.com" class="auth_mail" title="E-mail the corresponding author ; yucaihe@cczu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Zhi-Cheng Taoa ; Jun-Hua Dia ; Liang Chena ; Lin-Bing Zhanga ; Dan-Ping Zhanga ; Gang-Gang Chonga ; Feng Liua ; Yun Dinga ; Chun-Xia Jianga ; Cui-Luan Maa ; c
- 关键词：Rice straw ; Dilute NaOH pretreatment ; Ethyl 4-chloro-3-oxobutanoate ; Ethyl (R)-4-chloro-3-hydroxybutanoate ; E. coli CCZU-A13
- 刊名：Bioresource Technology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：214
- 期：Complete
- 页码：411-418
- 全文大小：1638 K
文摘
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Hydrolyzates promoted initial reaction rate and intracellular NADH content.
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Emulsifier OP-10 (20 mM) increased the initial reaction rate of whole cells.
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Hp-β-cyclodextrin (0.010 mol Hp-β-CD/mol COBE) increased the initial reaction rate.
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Effective biosynthesis of (R)-CHBE (800 mM) was in [Bmim]PF6-hydrolyzate media.