Highly efficient synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate and its derivatives by a robust NADH-dependent reductase from E. coli CCZU-K14
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- 作者：Yu-Cai Hea ; b ; heyucai2001@163.com" class="auth_mail ; yucaihe2007@aliyun.com" class="auth_mail ; Zhi-Cheng Taoa ; Xian Zhangb ; Zhen-Xing Yanga ; Jian-He Xub
- 关键词：Asymmetric reduction ; Biotransformation ; Ethyl 4-chloro-3-oxobutanoate ; Ethyl (S)-4-chloro-3-hydroxybutanoate ; Recombinant E. coli CCZU-K14
- 刊名：Bioresource Technology
- 出版年：June 2014
- 年：2014
- 卷：161
- 期：Complete
- 页码：461-464
- 全文大小：396 K
文摘
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Reductase (CmCR) from Candida magnoliae was discovered by genome mining.
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Recombinant E. coli CCZU-K14 was employed for efficiently reducing COBE.
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COBE at 3 M was reduced to (S)-CHBE (>99.9% ee; yield > 99%) after 14 h.
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E. coli CCZU-K14 could synthesize (S)-CHBE and its derivatives (>99.9% ee).