A liposome-encapsulated spin trap for the detection of nitric oxide
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- 作者：Donald J. Hirsha ; ; Brittany M. Schielerb ; Katherine M. Fomchenkoa ; Ethan T. Jordanc ; Kay D. Bidleb
- 关键词：BH4 ; (6R)-5 ; 6 ; 7 ; 8-Tetrahydrobiopterin dihydrochloride ; DPPG ; 1 ; 2-dipalmitoyl-sn-glycero-3-phospho-(1&prime ; -rac-glycerol) ; DTC-Fe ; dithiocarbamate-iron complex ; DTT ; dithiothreitol ; EDTA ; ethylenediaminetetraacetic acid ; dipotassium salt dihydrate ; EPR ; electron paramagnetic resonance ; FAT-MLVs ; frozen-and-thawed multi-lamellar vesicles ; HEPES ; 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid ; iNOS ; recombinant inducible nitric oxide synthase from mouse ; LEST ; liposome-encapsulated spin-t
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：96
- 期：Complete
- 页码：199-210
- 全文大小：1708 K
文摘
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A method for the encapsulation of a dithiocarbamate-iron spin trap is described.
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Encapsulation prevents enzyme inhibition.
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20">Encapsulation reduces abiotic nitric oxide production.
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25">Kinetics of nitric oxide production can be followed over minutes or hours.
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Limit of detection is 40 nM in 500 μL.