A multi-generation Calanus finmarchicus culturing system for use in long-term oil exposure experiments
详细信息 查看全文
- 作者：Louise Kiel Jensen ; JoLynn Carroll ; Gunnar Pedersen ; Ketil Hylland ; Salve Dahle and Torgeir Bakke
- 关键词：Arctic ; Calanus finmarchicus ; Culturing ; Egg production ; Development ; Pollution
- 刊名：Journal of Experimental Marine Biology and Ecology
- 出版年：2006
- 出版时间：30 May 2006
- 年：2006
- 卷：333
- 期：1
- 页码：71-78
- 全文大小：145 K
文摘
Crustacean zooplankton form the keystone link between primary producers and fish stocks along the Norwegian shelf and in the southern part of the Barents Sea. We have established a multi-generation cultivation system for zooplankton in order to perform future experiments on the biological effects of drilling discharges from the offshore petroleum industry. A population of the cold-water species, Calanus finmarchicus, was collected in March 2004 and maintained in a static system of 100 l polypropylene containers through all stages (Eggs–CVI). The population exhibited an average developmental time of 105 days which corresponds to realistic sub-Arctic to Arctic conditions (water temperatures = 5 °C (spring) and 8 °C (late summer)). A series of experiments was performed to examine copepod egg production as a function of different food sources and feeding concentrations. Only minor differences in egg production were observed for C. finmarchicus females fed with varying concentrations of the two diatom species Chaetoceros socialis and Thalassiosira weissflogii. In a separate experiment, the response to food availability was examined over time by tracking egg production and fecal pellet production (used as an indicator of food ingestion). Both hatching success and food ingestion were positively correlated with the food concentrations offered to adult females. Through this work we have demonstrated that C. finmarchicus populations can be maintained in the laboratory through multiple generations. In addition, methods to control egg production through changes in food availability have been established making it feasible to control the start date of exposure experiments or the timing of the collection of eggs to initiate a new generation.