Species-dependent sensitivity to contaminants: An approach using primary hepatocyte cultures with three marine fish species

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• 作者：Kathrin Sabine  ; Ellesat ;   ; ^{kathrin.ellesat@bio.uio.no} ; Mazyar  ; Yazdani ;   ; ^{mazyar.yazdani@bio.uio.no} ; Tor Fredrik  ; Holth ;   ; ^{t.f.holth@bio.uio.no} ; Ketil  ; Hylland  ; ;   ; ^{ketilhy@bio.uio.no}
• 关键词：Species differences ; Effects-fish ; Hepatocytes ; Culture ; Cytotoxicity ; Statin ; Copper ; Metals ; Lipid
• 刊名：Marine Environmental Research
• 出版年：2011
• 出版时间：October 2011
• 年：2011
• 卷：72
• 期：4
• 页码：216-224
• 全文大小：1106 K

文摘

There is limited knowledge about the sensitivity of different fish species to environmental pollutants. Such information is pivotal in risk assessment and to understand why some species appear to be more tolerant to contaminants than others. The aim of the current study was to evaluate whether primary hepatocyte cultures of three marine fish species could be established in the field and whether their sensitivity to selected contaminants would differ. Primary hepatocyte cultures of three marine fish species (plaice, long rough dab, Atlantic cod) were established and exposed for 24?h to copper (20?500?mg?L^?) and statins (1?00?mg?L^?). Endpoints were esterase activity, metabolic activity and reduced glutathione (GSH) content, all using fluorescent probes. Flatfish hepatocytes were more susceptible to copper and statin exposure than hepatocytes from cod. This study has shown that species-dependent differences in contaminant sensitivity can be investigated using primary hepatocyte cultures.