Exploring diagnostic potentials of radioiodinated sennidin A in rat model of reperfused myocardial infarction

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• 作者：Cuihua Jiang^a ; Meng Gao^a ; Yue Li^a ; Dejian Huang^a ; Nan Yao^a ; Yun Ji^c ; Xuejiao Liu^a ; Dongjian Zhang^a ; Xiaoning Wang^a ; Zhiqi Yin^b ; Su Jing^d ; Yicheng Ni^a ; ^e ; Jian Zhang^a ; ^{zjwonderful@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sennidin A (PubChem CID ; 122839) ; Hypericin (PubChem CID ; 5281051)
• 刊名：International Journal of Pharmaceutics
• 出版年：2015
• 出版时间：10 November 2015
• 年：2015
• 卷：495
• 期：1
• 页码：31-40
• 全文大小：2341 K

文摘

Non-invasive “hot spot imaging” and localization of necrotic tissue may be helpful for definitive diagnosis of myocardial viability, which is essential for clinical management of ischemic heart disease. We labeled Sennidin A (SA), a naturally occurring median dianthrone compound, with ¹³¹I and evaluated ¹³¹I SA as a potential necrosis-avid diagnostic tracer agent in rat model of reperfused myocardial infarction. Magnetic resonance imaging (MRI) was performed to determine the location and dimension of infarction. ¹³¹I-SA was evaluated in rat model of 24-hour old reperfused myocardial infarction using single-photon emission computed tomography/computed tomography (SPECT/CT), biodistribution, triphenyltetrazolium chloride (TTC) histochemical staining, serial sectional autoradiography and microscopy. Gamma counting revealed high uptake and prolonged retention of ¹³¹I SA in necrotic myocardium and fast clearance from non-targeted tissues. On SPECT/CT images, myocardial infarction was persistently visualized as well-defined hotspots over 24 h, which was confirmed by perfect matches of images from post-mortem TTC staining and autoradiography. Radioactivity concentration in infarcted myocardium was over 9 times higher than that of the normal myocardium at 24 h. With favorable hydrophilicity and stability, radioiodinated SA may serve as a necrosis-avid diagnostic agent for assessment of myocardial viability.