Characterizing the mitochondrial DNA polymerase gamma interactome by BioID identifies Ruvbl2 localizes to the mitochondria

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• 作者：Sanduni U. Liyanage^a ; ^b ; Etienne Coyaud^a ; Estelle M.N. Laurent^a ; Rose Hurren^a ; Neil Maclean^a ; Stuart R. Wood^c ; ¹ ; Lawrence Kazak^c ; ² ; Aisha Shamas-Din^a ; Ian Holt^c ; Brian Raught^a ; Aaron Schimmer^a ; ^b ; ^{aaron.schimmer@utoronto.ca}
• 关键词：BioID ; proximity dependent biotin labelling
• 刊名：Mitochondrion
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：32
• 期：Complete
• 页码：31-35
• 全文大小：640 K
• 卷排序：32

文摘

Human mitochondrial DNA (mtDNA) is replicated by the mitochondrial DNA polymerase gamma (POLG). Using proximity dependent biotin labelling (BioID), we characterized the POLG interactome and identified new interaction partners involved in mtDNA maintenance, transcription, translation and protein quality control. We also identified interaction with the nuclear AAA + ATPase Ruvbl2, suggesting mitochondrial localization for this protein. Ruvbl2 was detected in mitochondria-enriched fractions in leukemic cells. Additionally, transgenic overexpression of Ruvbl2 from an alternative translation initiation site resulted in mitochondrial co-localization. Overall, POLG interactome mapping identifies novel proteins which support mitochondrial biogenesis and a potential novel mitochondrial isoform of Ruvbl2.