Porphyrins in urine after administration of 5-aminolevulinic acid as a potential tumor marker

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• 作者：Masahiro  ; Ishizuka^c ; Yuichiro  ; Hagiya^a ; Yasuhiro  ; Mizokami^b ; Kanako  ; Honda^b ; Kenji  ; Tabata^a ; Toshiaki  ; Kamachi^b ; Kiwamu  ; Takahashi^c ; Fuminori  ; Abe^c ; Tohru  ; Tanaka^c ; Motowo  ; Nakajima^c ; Shun-ichiro  ; Ogura PhD^a ;   ; ^{sogura@bio.titech.ac.jp} ; Ichiro  ; Okura^b
• 关键词：Aminolevulinic acid ; Uroporphyrin ; Coproporphyrin ; Tumor marker ; Urine
• 刊名：Photodiagnosis and Photodynamic Therapy
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：8
• 期：4
• 页码：328-331
• 全文大小：239 K

文摘

Summary

Background

Tumor markers are commonly used for cancer screening and as indicators of therapeutic effects. Certain types of tumor have been known to produce a variety of porphyrins after 5-aminolevulinic acid (ALA) administration. In this study, porphyrins in tumor-bearing mouse urine were analyzed after oral administration of ALA in order to identify new tumor markers excreted in the urine.

Methods

Porphyrin concentrations in the urine of tumor-bearing mice were measured after administration of 1.0 mg of ALA (approximately 50 mg kg^?).

Results

Porphyrin concentrations in the urine of tumor-bearing mice increased after administration of ALA. HPLC analysis of the urine revealed the existence of uroporphyrin (UP) and coproporphyrin (CP) in the urine of ALA-treated tumor-bearing mice. Furthermore, at 3 h after ALA administration, UP concentrations in the urine of tumor-bearing mice significantly increased compared to those in the urine of normal mice.

Conclusion

These results suggest that UP as a precursor of heme detected in the urine of tumor-bearing mice after ALA administration is a potential marker of tumor development.